Ribo-Seq, also referred to as ribosome profiling or ribosome footprinting, is a next-generation sequencing (NGS)-based transcriptomic research technique that measures translation comprehensively and quantitatively. Ribo-Seq detected the precise positions of ribosomes to determine which mRNAs are being actively translated, which allows for the profiling of the active ribosomes in a cell at a particular moment, as well as the location of the translation initiation sites, the complement of translated ORFs, the specific locations of ribosomes on the mRNA, and the translation rate of ribosomes.
| ribosome sequencing |
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CUT&Tag (Cleavage Under Targets and Tagmentation) Sequencing is a next-generation sequencing-based method for the study of protein-DNA interactions. CUT&Tag is a new generation of ChIP-Seq without suffering from a number of practical and economical limitations, for it does not require cells to be lysed nor chromatin to be fractionated.